GIBSONIA, Pa. – Three transgender students filed a federal lawsuit against the Pine-Richland School District this week after the school board changed the bathroom policy to segregate students by biological sex, rather than gender identity under an old policy.

The school board’s Sept. 12 resolution means transgender students in the district can no longer use whatever restroom they “identify” with and are now restricted to facilities that correspond to the organs God gave them, the Post-Gazette reports.

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The move infuriated three Pine-Richland High School students – two male students who feel like females and one female student who thinks she’s a male – and convinced them to file a lawsuit with the help of gay rights group Lambda Legal in Pittsburgh on Thursday.

The two transgender female students, Juliet Egancho and Elssa Ridenour, are both 18 years old, while the transgender male student identified in court records as A.S. is 17 years old. The lawsuit names the school district, as well as superintendent Brian R. Miller and high school principal Nancy Bowman, according to the news site.

“Forcing transgender students to use restrooms that don’t match their gender identity or forcing them to use separate single stall restrooms erases this student’s gender identity, endangers the health and safety of transgender students and isolates them from the entire student body,” Omar Gonzalez-Pagan, attorney for Lambda Legal, told CBS Pittsburgh.

The change comes after President Obama this spring issued a nationwide directive that threatened schools with the loss of federal education funding if they don’t comply with his interpretation of Title IX anti-discrimination laws.

The president believes federal law prohibits schools from “discriminating” against transgender students by forcing them to use the restroom and locker room facilities for their biological gender. U.S. schools must allow transgender students to use the facilities they choose, the president said, though dozens of states have sued the federal government to block the directive.

In August, a U.S. District judge in Texas overseeing a lawsuit involving 13 of those states imposed an injunction on Obama’s directive, forbidding the federal government from enforcing the president’s perspective, EAGnews reports.

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Regardless, Egancho contends the recent change in her school’s transgender policy means she no longer feels safe at school.

“I have so many other things to be thinking about,” she told KDKA. “I shouldn’t be thinking about where I have to go to the bathroom.”

The Pine-Richland lawsuit is one of dozens filed by transgender students against their schools over access to restrooms and locker room facilities that align with their gender identity.

In early August, the U.S. Supreme Court blocked a lower court ruling in one of the more high-profile cases that would have forced a Virginia school district to comply with Obama’s transgender directive.

The court ruled that Gloucester school district’s biologically based bathroom policy can remain in effect as transgender student Gravin Grimm’s discrimination lawsuit continues forward in the court, justices ruled in a 5-3 decision.